OLA1 Knockout cell line(LoVo)

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location： Home > Products > KO Cell Lines > OLA1 Knockout cell line(LoVo)

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OLA1 Knockout cell line(LoVo) Order

OLA1 Knockout cell line(LoVo)
Catalog#
YKO-HJ083
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
OLA1 Knockout cell line(LoVo)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
OLA1 Knockout cell line (LoVo)
Species
Human
Gene Name
OLA1
Gene ID
29789
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 5 (based on transcript OLA1-201)
Validation: PCR+Sanger Sequencing
OLA1 Knockout cell line(LoVo) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the OLA1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the LoVo cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, OLA1 gene knockout OLA1 Knockout cell line (LoVo) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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